First Demonstration of Phase Contrast Scanning Transmission Electron Microscopy
نویسندگان
چکیده
Biological molecules are composed of light elements and can be considered as phase objects for the electron waves. Therefore, images of their unstained specimens show low contrast in transmission electron microscopy (TEM). Phase contrast TEM (P-TEM) is one of the powerful techniques to enhance image contrast of the phase objects [1]. In P-TEM, the phase plate (PP) is placed at a back focal plane (BFP) of the objective lens (OL), and its role is giving a phase shift on a portion of transmitted electron waves, which are scattered or unscattered depending on the type of the PP.
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